It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent , genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study , genetic diversity in a . polytricha was higher than that in a . auricula : 4 in this study , a . fuscosuccinea had a higher homology to a . auricula than to a . polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a . auricular and a . fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a . auricula as a probe which hybridized with a . auricula and a . fuscosuccinea except a . polytricha , further confirming the veracity of the results from eric - pcr ; 7 in this study , isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity 本研究中,木耳屬2個(gè)種的2個(gè)菌株在its區(qū)域表現(xiàn)出較高的保守性, 4種限制型內(nèi)切酶的酶切圖譜沒(méi)有顯示出多態(tài)性;增加內(nèi)切酶種類及供試菌株數(shù)量,有可能獲得具有多態(tài)性的限制性內(nèi)切酶酶切圖譜; 9本實(shí)驗(yàn)中, its區(qū)域的真菌特異性引物與真核生物通用引物對(duì)于擴(kuò)增效果無(wú)較大差異,擴(kuò)增片段長(zhǎng)度均為650bp左右; 10根據(jù)形態(tài)學(xué)實(shí)驗(yàn)、 eric - pcr實(shí)驗(yàn)以及southern雜交實(shí)驗(yàn)的結(jié)果分析,紫木木耳屬種質(zhì)資源的遺傳鑒定和遺傳多樣性評(píng)價(jià)耳極有可能是毛木耳種的一個(gè)變種; n .本研究中所用的gutc法是一種適用于木耳屬菌株基因組洲a快速提取的方法; 12 .傳統(tǒng)的形態(tài)學(xué)分類法和現(xiàn)代的分子生物學(xué)分類法,兩者的關(guān)系是相輔相成,互為驗(yàn)證